Correlation of NUF2 Overexpression with Poorer Patient Survival in Multiple Cancers / Journal of the Korean Cancer Association, 대한암학회지

Purpose@#NUF2 has been implicated in multiple cancers recently, suggesting NUF2 may play a role in the common tumorigenesis process. In this study, we aim to perform comprehensive meta-analysis of NUF2 expression in the cancer types included in the Cancer Genome Atlas (TCGA). @*Materials and Methods@#RNA-sequencing data in 31 cancer types in the TCGA data and 11 independent datasets were used to examine NUF2 expression. Silencing NUF2 using targeting shRNAs in hepatocellular carcinoma (HCC) cell lines was used to evaluate NUF2’s role in HCC in vitro and in vivo. @*Results@#NUF2 up-regulation is significantly observed in 23 out of the 31 cancer types in the TCGA datasets and validated in 13 major cancer types using 11 independent datasets. NUF2 overexpression was clinically important as high NUF2 was significantly associated with tumor stages in eight different cancers. High NUF2 was also associated with significantly poorer patient overall survival and disease-free survival in eight and six cancers, respectively. We proceeded to validate NUF2 overexpression and its negative association with overall survival at the protein level in an independent cohort of 40 HCC patients. Compared to the non-targeting controls, NUF2 knockdown cells showed significantly reduced ability to grow, migrate into a scratch wound and invade the 8 μm porous membrane in vitro. Moreover, NUF2 knockdown cells also formed significantly smaller tumors than control cells in mouse xenograft assays in vivo. @*Conclusion@#NUF2 up-regulation is a common feature of many cancers. The prognostic potential and functional impact of NUF2 up-regulation warrant further studies.

Clinical features of traumatic optic neuropathy in 265 cases / 中华耳鼻咽喉头颈外科杂志

Objective@#To analyze the clinical features and the pathogenetic law of traumatic optic neuropathy through epidemiologic study.@*Methods@#265 cases (275 eyes) with TON treated in Department of Otorhinolaryngology Head and Neck Surgery, the Affiliated Hospital of Qingdao University from April 1999 to August 2015 were retrospectively analyzed. Multiple Logistic regression analyses were used to evaluate potential prognostic factors on visual acuity.@*Results@#TON occured mostly in young (194/265, 73.21%) man (235/265, 88.68%), the majority of patients came from villages and towns (209/265, 78.87%). Traffic accident (197/265, 74.34%) remained the main etiology, with strike (36/265, 13.58%) and fall (17/265, 6.42%) as the common etiology. Most patients had head injuries. The effective rate of vision improvement was 53.45%(147/275). Multiple logistic regression analyses identified that initial visual acuity with light perception or better vision, optic canal fracture and orbital wall fracture were visual acuity key factors of TON (χ2 value was 24.674, 19.755, 9.175, respectively, all P<0.01), initial visual acuity with light perception or better vision was the protective factor on visual acuity recovery (OR=5.008, P<0.001), the presence of optic canal fracture and orbital wall fracture were the risk factors on visual acuity recovery (OR value was 0.110, 0.329, respectively, all P<0.01).@*Conclusions@#Ton occurs mostly in young man because of traffic accident. Visual impairment of TON is severe. The suitable preventive measures should be carried out according to its epidemiological features.

Different intranasal corticosteroids effects on nasal airway remodeling of allergic rhinitis murine and matrix metalloproteinase-9 / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To discuss the effect of different doses intranasal corticosteroids on remodeling of allergic rhinitis (AR) mice nasal mucosa and expression level of matrix metalloproteinase-9 (MMP-9).@*METHOD@#Thirty BALB/c female mice were divided into five groups randomly and received OVA or normal saline (NS) with intraperitoneal injection or nasal challenge, respectively. The treatment groups received additional different doses of budesonide (0.6 μg/20 g, 3.0 μg/20 g and 15.0 μg/20 g) daily for 16 weeks. We assessed the nasal symptoms at 4 and 16 weeks. Collected the mice nasal tissue, and then stained with hematoxylin-eosin, Masson's Trichrome, and periodic acid-schiff respectively to evaluate airway remodeling at 16 weeks. MMP-9 was measured with enzyme-linked immunosorbent assay (ELISA). Result: Times of rubbing, sneezes and infiltrate of eosinophil increased more in B group than in A group, and subepithelial fibrosis, collagen deposition, goblet cell hyperplasia, and submucosal gland hypertrophy were only observed in B group at 16 weeks. The nasal symptoms and eosinophil infiltration were inhibited by treatment with budesonide from a dose of 0.6 μg onwards, while the prevention of structure changes was only observed with 3.0 μg onwards. In addition, intranasal budesonide reduced MMP-9 in the nasal of AR mice.@*CONCLUSION@#The study suggests that higher dose intranasal corticosteroids might inhibit the airway remodeling of nasal mucosa by reducing MMP-9.

Blood supply of the pedicle nasal septum mucosa flap of rabbit in experiment / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To explore the blood supply of the pedicle nasal septum mucosa flap of rabbit, in order to supply the theory and experiment basis for making the pedicle nasal septum mucosa flap to repair nasal cavity and skull base defect.@*METHOD@#Twenty rabbits corpses were induced into the experimental subject, and inject 5 ml blue ink into the external carotid artery, then longitudinal cut apart the middle head of rabbit, finally observe the blood supply of the pedicle nasal septum mucosa flap.@*RESULT@#The blood supply of the pedicle nasal septum mucosa flap mostly come from the vessels of extremitas anterior part of nasal septum.@*CONCLUSION@#Keep the he vessels of extremitas anterior part of nasal septum can guarantee the blood supply of mucous membrane, enhance the survival rate of nasal septum mucous membrane.

Endoscopic endonasal approach resection for anterior skull base, pterygopalatine fossa and infratemporal fossa tumors: retrospective analysis of 73 patients / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To summarize the clinical experience with endoscopic transnasal resection of nasal skull- base neoplasms, which involved anterior skull base, pterygopalatine fossa, nfratemporal fossa.@*METHOD@#Clinical data from 73 patients performed on endoscopic transnasal resection of nasal skull-base neoplasms were analyzed retrospectively.@*RESULT@#Total tumor removal was obtained in 54 cases, subtotal removal in 19 cases. In 16 cases of benign tumor, the postoperative survival rate was 100%; Malignancy in 57 cases, of which 16 patients were died, and half-year survival rate was 71.9%.@*CONCLUSION@#Endoscopic endonasal approach be able to fully reveal and re- moval of lesions involving the anterior skull base, pterygopalatine fossa and infratemporal fossa. The approach is feasible and safe.

The maxillary sinus morphology that affect the vision of nasal endoscopy in maxillary sinus surgery / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To observe the gasification of the maxillary sinus which might affect the vision of the maxillary sinus surgery with endoscope.@*METHOD@#The diameters of 2 000 maxillary sinus were observed and measured hy normal sinus CT scan. The maxillary sinus were then genotyped with the data.@*RESULT@#Morphology of the medial wall of maxillary sinus played an important role in maxillary sinus surgery. According to the horizontal position CT data, the middle part of maxillary sinus medial wall included two shapes: 857 triangle (85.7%) and 143 semicircular (14.3%). According to the coronal CT data, the middle part of maxillary sinus medial wall varied a lot, there were also two shapes of them: 341 convex type (31.1%) and 659 straight type (65.9%). In contrast, the former and back parts varied a little. Therefore, we genotyped the maxillary sinus according to the morphology of maxillary sinus medial wall and the gasification level. There were 3 types of our genotyping: 662 of I type (66.2%), 265 of II type (26.5%), 73 of III type (7.3%).@*CONCLUSION@#The medial wall of maxillary sinus should be considered before the surgery of nasal endoscopy because of the great affections of which to endoscopy version.

Computed tomography analysis of anatomic structure related to endoscopic axilla approach for surgery of frontal sinus / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To observe the CT imaging features of anatomic structure related to endoscopic axilla approach for surgery of the frontal recess and frontal sinus.@*METHOD@#Thirty patients without a history of frontal sinus disease were undergone 16 line high speed spiral computed tomography. The computed tomographic images were analyzed to measure the related structures.@*RESULT@#The vertical distance from the front attachment point of the middle turbinate to the skull base was 13.88 +/- 2.59 mm. The horizontal distance from the top point of the axilla of the middle turbinate to the anterior wall of the frontal sinus outflow tract was 5.77 +/- 12.32 mm, to the anterior wall of the nasal cavity was 13.67 +/- 12.54 mm, to the lamina papyracea or lacrimal sac was 5.89 +/- 1.69 mm.@*CONCLUSION@#Sixteen line high speed spiral computed tomography is helpful to endoscopic axilla approach for surgery of the frontal recess and frontal sinus.

The anatomy research of expanding vision of endoscopic maxillary sinus operation / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To get the anatomical method of expanding vision of endoscopic maxillary sinus operation to providing guidance for the satisfactory surgical vision by measurement of nasal cavity and maxillary sinus relative structure.@*METHOD@#By anatomical measurement from 30 human cranium preparations (21 male, 9 female), which were marinated by 10% formaldehyde solution, we tried to find out anatomical factors that influence expanding vision of endoscopic maxillary sinus operation.@*RESULT@#Measurement of cranium preparations: respective distance between bone nasolacrimal canal anterior wall superior extremity, central extremity, bottom extremity and borderline of maxillary sinus anterior wall and medial wall were 0 mm, (1.90 +/- l.03) mm, (3.29 +/- 1.04) mm. Distance between anterior inferior part of bone nasolacrimal canal and anterior edge of inferior concha was (5.13 +/- 0.62) mm, and vertical distance between anterior inferior part of bone nasolacrimal canal and nasal bottom was (16.89 +/- 0.97) mm. The difference between right and left side had no significant statistic sense (P > 0.05). Removal of the bone area: the upper and lower diameter of part of the inferior turbinate attachment was (9.43 +/- 1.72) mm, anteroposterior diameter from top to bottom was (9.76 +/- 0.83) mm, (11.39 +/- 0.50) mm, (12.85 +/- 0.66) mm, the upper and lower diameter of the following part of the inferior turbinate attachment was (13.52 +/- 0.83) mm, anteroposterior diameter from top to bottom was (19.89 +/- 1.37) mm, (16.59 +/- 0.77) mm, (12.48 +/- 0.91) mm.@*CONCLUSION@#Dissection from inferior turbinate anterior part approach to maxillary sinus could expand the endoscopic operation vision.

Endoscopic feasibility study and nasal septum median path of frontal sinus surgery / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To discuss the feasibility of endoscopic frontal sinus surgery in the nasal septum median path.@*METHOD@#(1) Sixty adult cadaveric heads fixed with formalin were CT scanned,and were three dimensional reconstruction. (2) Thirty adult cadaveric heads were sawn along the sagittal line close to the side of the nasal septum, then the important anatomic marks were observed and measured. (3) Combined with CT and anatomical data, thirty adult cadaveric heads were operated in different degree, and the damage of nasal septum and fila olfactoria were detected in the same time.@*RESULT@#(1) The roots of middle nasal concha were simulated in the endoscopic frontal sinus surgery. The operation time, operative procedures, markers foundation, endoscopic back of posterior border of frontal sinus foundation and attached to the symphysis with cribriform plate and the top of ethmoidal sinus were recorded. (2) The intersection point formed by the level of middle nasal concha and the vertical of middle nasal concha corresponded with the nasal septum was called the M point. The distance from the M point to the horizon of the nasal bone was (20.07 +/- 6.21) mm, the distance from the M point to the first fila olfactoria was (24.38 +/- 7.68) mm, the distance from the first fila olfactoria to the posterior edge of frontal sinus was (9.57 +/- 2.73) mm, the distance from the root of the middle nasal concha to posterior edge of frontal sinus was (5.38 +/- 1.23) mm, the anteroposterior diameter of frontal sinus fundus was (7.62 +/- 2.45) mm, the transverse diameter of frontal sinus fundus was (9.41 +/- 3.37) mm, the seesaw diameter of frontal sinus partition was (16.97 +/- 3.23) mm, the anteroposterior diameter of frontal sinus partition was (12.34 +/- 2.23) mm. (3) The operation time through the nasal septum path was 105 minutes which combined with CT and anatomical measurements. 0 degrees endoscopy could be used to observe the frontal part of the lateral, posterior and top wall, while nasal septum remove should be finished with 30 degree endoscopy. The bottom of frontal sinus can be exposed and removed with 0 degree endoscopy. 3 cases of cadaveric frontal sinus lateral wall can not be observed with 70 degree endoscopy. 30 cases of cadaveric frontal sinus,some of the top and the lateral wall, anterior and posterior wall could be observed with 70 degree endoscopy, nasal septum damage range was about 2.23 cm x 2.59 cm, and no fila olfactoria damage was found.@*CONCLUSION@#Endoscopic frontal sinus surgery in the nasal septum median path is a good way to find frontal sinus.

Feasibility of staged allogeneic larynx transplantation in dogs / 中国组织工程研究

BACKGROUND: The key to success in laryngeal transplantation is to control the infection. In practice, how to minimize the risk of infection should be considered first, so that the laryngeal allograft can survive after revascularization under the immunosuppressive state. Then the therapeutics methods should be focused on the functional recovery of respiratory, swallowing and phonation.OBJECTIVE: To explore the feasibility of staged allogeneic larynx transplant for dogs in order to decrease the infection rate.METHODS: Ten male conspecific Beagle dogs were assigned into five pairs of donor and receptor according to body mass and the size of larynx. The allogeneic larynx was embedded under the neck skin by allotransplantation. The dogs were treated with immunosuppressant daily to resist immnological rejection. The pharynx and trachea were contraposed after larynx survived to establishⅡstage laryngeal transplantation animal model.RESULTS AND CONCLUSION: InⅠstage of laryngeal transplantation, the laryngeal allograft survived well, blood supply was passable. Receptor dog 1 with vertical midline incision in the neck, receptor dogs 4 and 5 with transverse incisions in the neck grew well. No infection phenomena was found in receptor dogs 4 and 5. The lastⅡstage laryngeal transplantation was performed in the 4th week afterⅠstage laryngeal transplantation. Skin flap necrosis occurred in receptor dogs 2 and 3 with U-shape incisions. The donor larynx had different degree infection. Laryngeal allograft of receptor dog 2 died; laryngeal allograft of receptor dog 3 survived after treatment. The survival period of donor larynx in receptors was as long as 4 weeks. These findings indicate that the larynx infection is avoid or reduced by staged larynx transplantation with pre-embed laryngeal allograft after total laryngectomy. The receptor dog is tolerant; the donor larynx is easy to survive. The main histological change occurs in the epithelial mucosae which can be used as an inspect index of immunosuppressive agents. However, glandular organ secretion is a troublesome problem and immunodepressant must be continuously administrated for a long time.

Clinical analysis of rhinogenous ophthalmocele / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the diagnosis and treatment of rhinogenous ophthalmocele, to reduce misdiagnosis and missed diagnosis rate.@*METHOD@#Sixty-two patients with rhinogenous ophthalmocele disease in our hospital from January 1998 to October 2005 were retrospectively analyzed.@*RESULT@#Sixty-two patients with rhinogenous ophthalmocele disease, 18 frontal-ethmoid sinus cystic, 12 nasosinusitis, 6 fungal frontal sinusitis, 10 maxillary sinus carcinoma, 4 olfactory nerve tumour, 6 traumatic carotid cavernous fistula, 6 ethmoidal sinus osteoma. The rhinogenous ophthalmocele caused by frontal-ethmoid sinus cystic and nasosinusitis and ethmoidal sinus osteoma were cured. The maxillary sinus carcinomas were followed up for 5 years, 2 examples are alive, 2 examples are lost. The olfactory nerve tumours were death in 2 years after operation. The 2 traumatic carotid cavernous fistula were death, 4 were cured.@*CONCLUSION@#The disease with rhinogenous ophthalmocele was easily misdiagnosed for the low incidence of the disease. According to eyes reasons of ophthalmocele, choose the suitable operational method, it is satisfied with the result of treatment.